Recombinant Protein;Human PD-1 / PDCD1 Protein (HPLC-verified);100UG;Human PD-1, His Tag (PD1-H5221) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # NP_005009.2).;This protein carries a polyhistidine tag at the C-terminus, and has a calculated MW of 16.8 kDa. The N-terminus Sequence Analysis is Leu 25. The reducing (R) protein migrates as 31-44 kDa in SDS-PAGE due to glycosylation.;Immobilized Human PD-1, His Tag (Cat. No. PD1-H5221) at 0.2μg/mL (100 μL/well) can bind Human PD-L1, Fc Tag (Cat. No. PD1-H5258) with a linear range of 0.31-1.25 μg/mL (QC tested).;Immobilized Human PD-1, His Tag (Cat. No. PD1-H5221) at 0.2 μg/mL ( 100 μl/well ) can bind Human PD-L2, Fc Tag (Cat. No. PD2-H5251) with a linear range of 0.16-2.5 μg/mL (QC tested).;Opdivo(Nivolumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human PD-1, His Tag (Cat. No. PD1-H5221) with an affinity constant of 4.94 nM as determined in a SPR assay (Biacore T20