R&D Systems™ Recombinant Human PD-L1/B7-H1 Fc Chimera Biotin Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Human PD-1 Fc Chimera (Catalog # 1086-PD ) is immobilized at 1 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant PD-L1/B7-H1 Fc Chimera (Catalog# BT156) that produces 50% of the optimal binding response is 10-60 ng/mL.

Specifications

• Accession Number: Q9NZQ7
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 29126
• Molecular Weight (g/mol): M.W. (Observed): 70-75 kDa, reducing conditions; M.W. (theoretical): 52 kDa
• Quantity: 50 μg
• Source: Mouse myeloma cell line, NS0-derived human PD-L1/B7-H1 protein Human PD-L1 (Phe19-Thr239) (N-terminus) Accession # Q9NZQ7 DIEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Regulatory Status: RUO
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Specific Reactivity: PD-L1
• Conjugate: Biotin
• Species: Human
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: PD-L1