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R&D Systems™ Recombinant Human PD-L1/B7-H1 His Alexa Fluor™ 647 Protein

Catalog No. AFR9049020
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20 μg
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AFR9049020 20 μg
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His-tag

Measured by flow cytometry for its ability to bind anti-human PD-L1/B7-H1 Monoclonal Antibody conjugated beads. The concentration of Recombinant Human PD-L1/B7-H1 His-tag Alexa Fluor™ 647 (Catalog # AFR9049) that produces 50% of the binding response is 0.500-5.00 ng/mL.

Specifications

Accession Number Q9NZQ7.1
For Use With (Application) Bioactivity
Formulation Supplied as a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.
Gene ID (Entrez) 29126
Molecular Weight (g/mol) M.W.-observed: 31-42 kDa, under reducing conditions., M.W.-theroretical: 26 kDa
Quantity 20 μg
Source Human embryonic kidney cell, HEK293-derived human PD-L1/B7-H1 protein Phe19-Thr239, with a C-terminal 6-His tag
Storage Requirements Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after opening. 3 months, -20°C to -70°C under sterile conditions after opening.
Endotoxin Concentration <1.0 EU / 1 μg of the protein by the LAL method.
Gene Alias Avelumab, B7-H, B7H1, B7-H1, B7H1PDCD1L1, CD274, CD274 antigenMGC142294, CD274 molecule, PDCD1L1, PDCD1LG1, PDCD1LG1MGC142296, PDL1, PD-L1, PD-L1B7 homolog 1, PDL1PDCD1 ligand 1, programmed cell death 1 ligand 1, Programmed death ligand 1
Conjugate Alexa Fluor 647
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein PD-L1
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