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R&DSystems™ Recombinant Human PDGF-AA (CHO-expressed) Protein, CF

Catalog No. 11564PA010
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Catalog No. Quantity
11-564-PA010 10 μg
11-564-PA01M 1 mg
11-564-PA100 100 μg
11-564-PA050 50 μg
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Description

Recombinant Protein

Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.400-4.00 ng/mL.

Specifications

Accession Number NP_001382295.1
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with Trehalose.
Gene ID (Entrez) 5154
Molecular Weight (g/mol) M.W.-observed: 15-17 kDa & 20-22 kDa, M.W.-theroretical: 13 kDa
Quantity 10 μg
Source Chinese Hamster Ovary cell line, CHO-derived human PDGF-AA protein Ser87-Arg196
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Endotoxin Concentration <0.10 EU per 1 μg of the protein by the LAL method.
Gene Alias PDGF1, PDGF-A, PDGFAA
Conjugate Unconjugated
Reconstitution Reconstitute at 100 μg/mL in 4mL HCl.
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein PDGF-AA
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