R&D Systems™ Recombinant Human Plexin B1 (Sema domain) His Protein

Description

Recombinant Protein

Measured by its binding ability in a functional ELISA. When Recombinant Human Plexin B1 His-tag is coated at 1.00 μg/mL (100 μL/well), Recombinant Human Semaphorin 4D Fc Chimera (Catalog # 7470-S4 ) binds with an ED50 of 80.0-480 ng/mL.

Specifications

• Accession Number: O43157.3
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 5364
• Molecular Weight (g/mol): M.W.-observed: 60-68 kDa, under reducing conditions, M.W.-theroretical: 56 kDa
• Quantity: 50 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human Plexin B1 protein Leu20-Gln535, with a C-terminal 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: plexin B1, PLXN5, PLXNB1, SEP, Transmembrane Protein SEP
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 1.00 mg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Plexin B1