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R&D Systems™ Recombinant Human Plexin B1 (Sema domain) His Protein

Catalog No. 10803PB050
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10803PB050 50 μg
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Recombinant Protein

Measured by its binding ability in a functional ELISA. When Recombinant Human Plexin B1 His-tag is coated at 1.00 μg/mL (100 μL/well), Recombinant Human Semaphorin 4D Fc Chimera (Catalog # 7470-S4 ) binds with an ED50 of 80.0-480 ng/mL.

Specifications

Accession Number O43157.3
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Gene ID (Entrez) 5364
Molecular Weight (g/mol) M.W.-observed: 60-68 kDa, under reducing conditions, M.W.-theroretical: 56 kDa
Quantity 50 μg
Source Chinese Hamster Ovary cell line, CHO-derived human Plexin B1 protein Leu20-Gln535, with a C-terminal 6-His tag
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
Endotoxin Concentration <0.10 EU / 1 μg of the protein by the LAL method.
Gene Alias plexin B1, PLXN5, PLXNB1, SEP, Transmembrane Protein SEP
Conjugate Unconjugated
Reconstitution Reconstitute at 1.00 mg/mL in PBS.
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein Plexin B1
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