R&DSystems™ Recombinant Human R-Spondin 3 Fc Chimera Protein, CF

Catalog No.	Quantity
11-506-RS500	500 μg
11-506-RS100	100 μg
11-506-RS050	50 μg
11-506-RS020	20 μg

Catalog No. 11-506-RS500 Supplier R&DSystems™ Supplier No. 11506RS500

Description

Recombinant Protein

Measured by its ability to activate TCF reporter activity in HEK293 human embryonic kidney cells in the presence of Recombinant Human Wnt-3a (Catalog # 5036-WN ). The ED50 for this effect is 0.500-10.0 ng/mL.

Specifications

• Accession Number: Q9BXY4.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 84870
• Molecular Weight (g/mol): M.W.-observed: 53-69 kDa, under reducing conditions., M.W.-theroretical: 46 kDa
• Quantity: 500 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human R-Spondin 3 protein Human RSPO3 (Met33-Gly209) (N-terminus) Accession # Q9BXY4.1 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method.
• Gene Alias: Cristin 1, CRISTIN1, FLJ14440, hPWTSR, hRspo3, Protein with TSP type-1 repeat, PWTSR, Roof plate-specific spondin-3, RSPO3, RSpondin 3, R-spondin 3 homolog (Xenopus laevis), R-spondin-3, Thrombospondin type-1 domain-containing protein 2, thrombospondin, type I, domain containing 2, THSD2
• Conjugate: Unconjugated
• Reconstitution: Reconstitute the 20 μg size at 200 μg/mL in PBS. Reconstitute all other sizes at 500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: R-Spondin 3