R&D Systems™ Recombinant Human Semaphorin 3G Fc Chimera Protein

Description

Recombinant Protein

Measured by the ability to inhibit the proliferation of HUVECumbilical vein endothelial cells. The ED50 for this effect is 100-600 ng/mL.

Specifications

• Accession Number: Q9NS98.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 56920
• Molecular Weight (g/mol): M.W.-observed: 105-125 kDa, under reducing conditions, M.W.-theroretical: 111 kDa
• Quantity: 25 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human Semaphorin 3 G protein Human Semaphorin 3 G (Gly23-Thr782)(R557S, R558S, R560S, R561S, R774S, R777S) (N-terminus) Accession # Q9NS98.1 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: (semaphorin) 3 G, FLJ00014, MGC119473, sem2, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, SEMA3G, Semaphorin sem2, semaphorin-3 G
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: SEMA3G