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R&D Systems™ Recombinant Human Semaphorin 3G Fc Chimera Protein

Catalog No. 10804S3025
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25 μg
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10804S3025 25 μg
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Recombinant Protein

Measured by the ability to inhibit the proliferation of HUVECumbilical vein endothelial cells. The ED50 for this effect is 100-600 ng/mL.

Specifications

Accession Number Q9NS98.1
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Gene ID (Entrez) 56920
Molecular Weight (g/mol) M.W.-observed: 105-125 kDa, under reducing conditions, M.W.-theroretical: 111 kDa
Quantity 25 μg
Source Chinese Hamster Ovary cell line, CHO-derived human Semaphorin 3 G protein Human Semaphorin 3 G (Gly23-Thr782)(R557S, R558S, R560S, R561S, R774S, R777S) (N-terminus) Accession # Q9NS98.1 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
Endotoxin Concentration <0.10 EU / 1 μg of the protein by the LAL method.
Gene Alias (semaphorin) 3 G, FLJ00014, MGC119473, sem2, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, SEMA3G, Semaphorin sem2, semaphorin-3 G
Conjugate Unconjugated
Reconstitution Reconstitute at 500 μg/mL in PBS.
Purity or Quality Grade >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein SEMA3G
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