R&D Systems™ Recombinant Human SIRP alpha/CD172a Fc Biotin Protein

Description

Biotinylated via amines

Measured by its binding ability in a functional ELISA. When Recombinant Human CD47 Fc Chimera (Catalog # 4670-CD ) is immobilized at 0.1 μg/mL (100 μL/well), Biotinylated Recombinant SIRP alpha/CD172a Fc Chimera (Catalog # BT4546B) binds with an ED50 of 6-48 ng/mL.

Specifications

• Accession Number: NP_542970
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 140885
• Molecular Weight (g/mol): M.W. (Observed): 70-105 kDa, under reducting conditions; M.W. (theoretical): 64 kDa (monomer)
• Quantity: 50 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human SIRP alpha/CD172a protein Human SIRP alpha/CD172a (Gly27-Arg370) & (Glu31-Arg370) (N-terminus) Accession # NP_542970 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -70 °C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Conjugate: Biotin
• Species: Human
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: SIRP alpha/CD172a