R&D Systems™ Recombinant Human Sonic Hedgehog/Shh Protein, High Activity

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

The Recombinant Human Sonic Hedgehog/Shh Protein, High Activity is derived from HEK293. The Recombinant Human Sonic Hedgehog/Shh Protein, High Activity has been validated for the following applications: Bioactivity.

Specifications

• Accession Number: Q15465
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 6469
• Molecular Weight (g/mol): Observed M.W.: 18-24kDa, reducing conditions, Theoretical M.W.: 20kDa
• Quantity: 5μg
• Source: Human embryonic kidney cell,HEK293-derived human Sonic Hedgehog/Shh protein Cys24-Gly197
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70° C as supplied. 3 months, -20 to -70° C under sterile conditions after opening.
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Gene Alias: HHG1, HHG-1, HLP3, HPE3, MCOPCB5, MCOPCB5sonic hedgehog (Drosophila) homolog, Shh, ShhNC, SMMCI, SMMCIsonic hedgehog homolog (Drosophila), sonic hedgehog, sonic hedgehog homolog, sonic hedgehog protein, TPT, TPTPS
• Common Name: Sonic Hedgehog/Shh
• Gene Symbol: SHH
• Biological Activity: Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465. The ED50 for this effect is typically 6-36ng/mL.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Expression System: Human embryonic kidney cell, HEK293-derived Cys24-Gly197
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining