R&D Systems™ Recombinant Human Thioredoxin-1 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity

Measured by its ability to catalyze the reduction of insulin. The reaction leads to precipitation, which can be measured by absorbance at 650 nm. The specific activity is >10 A650/min/mg, as measured under the described conditions.

Specifications

• Accession Number: P10599
• For Use With (Application): Enzyme Activity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT.
• Gene ID (Entrez): 7295
• Molecular Weight (g/mol): M.W. (Observed): 12 kDa, reducing conditions; M.W. (theoretical): 12 kDa
• Name: Thioredoxin-1
• Quantity: 500 μg
• Source: E. coli-derived human Thioredoxin-1 protein Val2-Val105
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: TXN
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 1000 μg/mL in sterile PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Thioredoxin-1