R&D Systems™ Recombinant Human Thrombopoietin/TPO Protein, Animal-Free

Catalog No.	Quantity
BTTPOAFL050	50 μg
BTTPOAFL01M	1 mg

Catalog No. BTTPOAFL050 Supplier R&D Systems™ Supplier No. BTTPOAFL050

Description

Recombinant Protein

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The ED50 for this effect is 0.050-0.500 ng/mL. The specific activity of Recombinant Human Thrombopoietin is >1 x 107 units/mg, which is calibrated against the human Thrombopoietin reference standard (NIBSC code: 03/124).

Specifications

• Accession Number: NP_000451.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with Trehalose.
• Gene ID (Entrez): 7066
• Molecular Weight (g/mol): M.W.-observed: 18-19 kDa, under reducing conditions., M.W.-theroretical: 19 kDa
• Quantity: 50 μg
• Source: E. coli-derived human Thrombopoietin/Tpo protein Ser22-Leu195 Produced using non-animal reagents in an animal-free laboratory.
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method.
• Gene Alias: Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, megakaryocyte stimulating factor, MGDF, MGDFC-mpl ligand, MKCSF, MK-CSF, ML, MPL ligand, MPLLG, MPLLGMGC163194, Myeloproliferative leukemia virus oncogene ligand, THCYT1, THPO, thrombopoietin, thrombopoietin nirs variant 1, Tpo, TPOMKCSF
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 500 μg/mL in water.
• Purity or Quality Grade: Antigen Affinity-purified
• Protein: Thrombopoietin/THPO