R&D Systems™ Recombinant Human Tissue alpha-L-Fucosidase/FUCA1

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its ability to cleave a fluorogenic substrate 4-methylumbelliferyl-alpha-L-fucopyranoside. The specific activity is >4,800 pmol/min/μg, as measured under the described conditions.

Specifications

• Accession Number: P04066
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris and NaCl.
• Gene ID (Entrez): 2517
• Molecular Weight (g/mol): M.W. (Observed): 55-60 kDa, reducing conditions; M.W. (theoretical): 51 kDa
• Name: Tissue alpha-L-Fucosidase/FUCA1
• Quantity: 10 μg
• Source: Mouse myeloma cell line, NS0-derived human Tissue alpha-L-Fucosidase/FUCA1 protein Gln32-Lys466 with a C-terminal 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: FUCA1
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain (at 5 μg / lane.)
• Protein: Tissue alpha-L-Fucosidase/FUCA1