R&D Systems™ Recombinant Human VISTA Fc Chimera Biotinylated Protein

Catalog No.	Quantity
BT7126050	50 μg

Catalog No. BT7126050 Supplier R&D Systems™ Supplier No. BT7126050

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Human VSIG-3 Fc Chimera (Catalog # 9229-VS ) is immobilized at 5 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant VISTA/B7-H5/PD-1H Fc Chimera that produces 50% of the optimal binding response is 1-6 μg/mL. Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is typically 1-6 μg/m

Specifications

• Accession Number: AAH20568
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 64115
• Molecular Weight (g/mol): M.W. (Observed): 64 - 75 kDa, reducing conditions; M.W. (theoretical): 45 kDa (unlabeled)
• Quantity: 50 μg
• Source: Mouse myeloma cell line, NS0-derived human VISTA/B7-H5/PD-1 H protein Human VISTA/B7-H5/PD-1 H (Phe33-Ala194) Accession # AAH20568 IEGRMD Human IgG1 (Pro100-Lys330) N-terminμs
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Regulatory Status: RUO
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Conjugate: Biotin
• Cross Reactivity: VISTA/B7-H5/PD-1H
• Species: Human
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: VISTA/B7-H5/PD-1 H