R&D Systems™ Recombinant Human Wnt-2/sFRP-1 Complex Protein

Catalog No.	Quantity
11117WN010	10 μg

Catalog No. 11117WN010 Supplier R&D Systems™ Supplier No. 11117WN010

Description

Measured by its ability to activate Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 10.0-150 ng/mL.

Specifications

• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in MOPS and NaCl with Trehalose.
• Gene ID (Entrez): 7472
• Molecular Weight (g/mol): M.W.-observed: 30-45 kDa, under reducing conditions., M.W.-theroretical: 38 kDa (Wnt-2) & 33 kDa (sFRP-1)
• Quantity: 10 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human Wnt-2 protein Human Wnt-2 (Ser26-Thr360) (C-terminus) Accession # P09544.1 Human sFRP-1 (Ser32-Lys314) (C-terminus) Accession # AAB70793.1 (N-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: FrzA, INT1L1, INT1L1secreted growth factor, Int-1-like protein 1, Int-1-related protein, IRP, IRPprotein Wnt-2, SARP-2, sFRP1, wingless-type MMTV integration site family member 2, Wnt2
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 100 μg/mL in PBS.
• Purity or Quality Grade: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Protein: Wnt-2