R&D Systems™ Recombinant Mouse B7-H3 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its ability to enhance anti-CD3-induced proliferation of mouse CD3+ T cells. The ED50 for this effect is typically 1.5-7.5 μg/mL.

Specifications

• Accession Number: Q8VE98.1
• Characterization: Bioactivity
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Gene ID (Entrez): 80381
• Molecular Weight (g/mol): M.W. (Observed): 38-45 kDa, reducing conditions; M.W. (theoretical): 24.8 kDa
• Quantity: 1 mg
• Source: Mouse myeloma cell line, NS0-derived mouse B7-H3 protein Val29-Phe244, with a C-terminal 10-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: B7H3, B7H34Ig-B7-H3, B7-H3B7 homolog 3, CD276, CD276 antigen, CD276 molecule, Costimulatory molecule
• Gene Symbol: Cd276
• Product Type: Recombinant Protein
• Conjugate: Unconjugated
• Species: Mouse
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS containing (at least 0.1% human or bovine serum albumin.)
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: B7-H3/CD276