R&D Systems™ Recombinant Mouse B7-H4 Fc Chimera Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. Human T lymphocytes cultured for 72 hours with PHA were incubated for an additional 3 days in 96 well plates coated with 500 ng/mL anti-CD3 and Recombinant Mouse B7-H4 Fc Chimera. The ED50 for this effect is 0.5-3 μg/mL. Optimal dilutions should be determined by each laboratory for each application.

Specifications

• Accession Number: AAP37284
• Characterization: Bioactivity
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 79679
• Molecular Weight (g/mol): M.W. (Observed): 75-85 kDa, reducing conditions; M.W. (theoretical): 52.4 kDa (monomer)
• Quantity: 1 mg
• Source: Mouse myeloma cell line, NS0-derived mouse B7-H4 protein Mouse B7-H4 (Phe29-Pro258) (N-terminus) Accession # AAP37284 IEGRMDP Mouse IgG2a (Glu98-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.01 EU / 1 μg of the protein by the LAL method.
• Gene Alias: B7h.5, B7H4, B7-H4, B7H4T-cell costimulatory molecule B7x, B7S1, B7S1VCTN1, B7x, B7XPRO1291, FLJ22418, Immune costimulatory protein B7-H4, Protein B7S1, T cell costimulatory molecule B7x, V-set domain containing T cell activation inhibitor 1, V-set domain-containing T-cell activation inhibitor 1, Vtcn1
• Gene Symbol: Vtcn1
• Product Type: Recombinant Protein
• Conjugate: Unconjugated
• Species: Mouse
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: B7-H4