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R&D Systems™ Recombinant Mouse EphA2 Fc Chimera Protein

Catalog No. 639A2200
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200 μg
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639A2200 200 μg
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Extensive quality control produces lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Binding Activity

Measured by its ability to bind Recombinant Mouse Ephrin-A1 Fc Chimera (Catalog # 602-A1 ) in a functional ELISA. Immobilized Recombinant Mouse EphA2 Fc Chimera at 2 μg/mL (100 μL/well) can bind Recombinant Mouse Ephrin-A1 Fc Chimera with a linear range of 0.078-5 ng/mL. Optimal dilutions should be determined by each laboratory for each application.

Specifications

Accession Number AAA82113
For Use With (Application) Binding Activity
Formulation Lyophilized from a 0.2 μm filtered solution in Tris-HCl and EDTA.
Gene ID (Entrez) 1969
Molecular Weight (g/mol) M.W. (Observed): 105 kDa, reducing conditions; M.W. (theoretical): 84 kDa (monomer)
Name EphA2 Fc Chimera
Quantity 200 μg
Source Mouse myeloma cell line, NS0-derived mouse EphA2 protein Mouse EphA2 (Ala22-Ala535) (N-terminus) Accession # AAA82113 IEGRMD Human IgG1 (Pro100-Lys330) 6-His tag (C-terminus)
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
Structural Form Disulfide-linked homodimer
Endotoxin Concentration <1.0 EU / 1 μg of the protein by the LAL method.
Gene Symbol Epha2
Conjugate Unconjugated
Recombinant Recombinant
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein EphA2
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