R&D Systems™ Recombinant Mouse Klotho beta Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with human FGF RIIIc. The ED50 for this effect is 0.2-1 μg/mL in the presence of Recombinant Human FGF-21 (Catalog # 2539-FG ) and Heparin.

Specifications

• Accession Number: NP_112457
• For Use With (Application): Bioactivity
• Formulation: Supplied as a 0.2 μm filtered solution in PBS, Glycerol and EDTA.
• Gene ID (Entrez): 152831
• Molecular Weight (g/mol): M.W. (Observed): 140 kDa, reducing conditions; M.W. (theoretical): 110.2 kDa
• Name: Klotho beta
• Quantity: 50 μg
• Source: Mouse myeloma cell line, NS0-derived mouse Klotho beta protein Phe53-Leu995, with a C-terminal 10-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 1 month, 2°C to 8°C under sterile conditions after opening.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: Klb
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Klotho beta