R&D Systems™ Recombinant Mouse OLT-2/TARM1 Fc Chimera Protein

Description

The Recombinant Mouse OLT-2/TARM1 Fc Chimera Protein is derived from NS0

• TARM-1 (T cell interacting, activating receptor on myeloid cells-1), also name OLT-2 (OSCAR-like Transcript 2), is a type 1 membrane protein expressed by neutrophils, inflammatory monocytes, macrophages, and dendritic cells.
• Mature mouse TARM-1 contains a 242 amino acid (aa) long extracellular domain with two Ig-like C2 domains, a 21 aa long transmembrane domain and a 9 aa long cytoplasmic domain.
• The extracellular domain of mouse TARM-1 shares 81.6% and 46.5% aa identity with rat and human TARM-1 respectively.
• TARM-1 associates with the ITAM bearing adaptor FcR gamma, but not with DAP10 or DAP12.
• TARM1 expression is also up-regulated by bone marrow-derived macrophages and dendritic cells following stimulation with TLR agonists in vitro.
• TARM1 receptor stimulation on macrophages and neutrophils co-stimulate the secretion of proinflammatory cytokines induced by TLR ligands, such as LPS.
• TARM1 Fc fusion protein inhibits anti-CD3 induced CD4 + cell activation, suggesting that TARM1 ectodomain interacts with an unidentified receptor on T cells to inhibit T cell activation.

Specifications

• Accession Number: B6A8R8
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 441864
• Molecular Weight (g/mol): 53 kDa
• Purification Method: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining
• Quantity: 50 μg
• Source:

  Mouse myeloma cell line, NS0-derived mouse OLT-2/TARM1 protein

  Mouse TARM-1 (Gln17-Ile257) Accession # B6A8R8IEGRMDPMouse IgG2a (Glu98-Lys330)N-terminus C-terminus

• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, ≤ -20°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 500 μg/mL in PBS
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS