R&D Systems™ Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated mouse T cells. The ED50 for this effect is 0.15-0.75 μg/mL.

Specifications

• Accession Number: Q9EP73
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 29126
• Molecular Weight (g/mol): M.W. (Observed): 75-85 kDa, reducing conditions; M.W. (theoretical): 51.3 kDa (monomer)
• Name: B7-H1/PD-L1 Fc Chimera
• Quantity: 100 μg
• Source: Mouse myeloma cell line, NS0-derived mouse PD-L1/B7-H1 protein Mouse PD-L1 (Phe19-Thr238) (N-terminus) Accession # Q9EP73 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Structural Form: Disulfide-linked homodimer
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: Cd274
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: PD-L1