R&D Systems™ Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein

Description

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED50 is 8 to 40 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt-3a.

Specifications

• Accession Number: NP_619624
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 284654
• Molecular Weight (g/mol): 27 kDa
• Name: Cristin 3, CRISTIN3, FLJ40906Roof plate-specific spondin-1, hRspo1, roof plate-specific spondin, RSPO, RSPO1, RSPONDIN, RSpondin 1, R-spondin homolog (Xenopus laevis), R-spondin1, R-spondin-1
• Quantity: 10 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse R-Spondin 1 protein Met1-Gln265
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Gene Symbol: Rspo1
• Conjugate: Unconjugated
• Species: Mouse
• Reconstitution: Reconstitute at 100 μg/mL in PBS.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS. *1 mg pack size (01M) is supplied as a 0.2 μm filtered solution in PBS.
• Endotoxin Level: <0.01 EU per 1 μg of the protein by the LAL method.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.