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R&D Systems™ Recombinant Mouse R-Spondin 2 Fc Chimera Protein, CF

Catalog No. 11567RS100
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100 μg
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Catalog No. Quantity
11-567-RS100 100 μg
11-567-RS050 50 μg
11-567-RS020 20 μg
11-567-RS01M 1 mg
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Catalog No. 11-567-RS100 Supplier R&D Systems™ Supplier No. 11567RS100
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Description

Recombinant Protein

Measured by its ability to activate TCF reporter activity in HEK293 human embryonic kidney cells in the presence of Wnt-3a. The ED50 for this effect is 0.600-12.0 ng/mL.

Specifications

For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Gene ID (Entrez) 340419
Molecular Weight (g/mol) M.W.-observed: 52-63 kDa, under reducing conditions., M.W.-theroretical: 46 kDa
Quantity 100 μg
Source Chinese Hamster Ovary cell line, CHO-derived mouse R-Spondin 2 protein Mouse RSPO-2 (Ala32-Gly205) (N-terminus) Accession # Q8BFU0.1 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Endotoxin Concentration <0.10 EU per 1 μg of the protein by the LAL method.
Gene Alias Cristin 2, CRISTIN2, hRspo2, MGC35555, MGC43342, Roof plate-specific spondin-2, RSPO2, RSpondin 2, R-spondin 2 homolog (Xenopus laevis), R-spondin-2
Conjugate Unconjugated
Reconstitution Reconstitute the 20 μg size at 200 μg/mL in PBS. Reconstitute all other sizes at 500 μg/mL in PBS.
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein R-Spondin 2
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