R&D Systems™ Recombinant Mouse Semaphorin 4A mFc Chimera Protein

Catalog No.	Quantity
11096S4050	50 μg

Catalog No. 11096S4050 Supplier R&D Systems™ Supplier No. 11096S4050

Description

Measured by its binding ability in a functional ELISA. When Recombinant Mouse Semaphorin 4A mFc Chimera (Catalog # 11096-S4) is immobilized at 2.0 μg/mL (100 μL/well), the concentration of Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera Protein (Catalog # 2078-T4 ) that produces 50% of the optimal binding response is 0.100-0.600 μg/mL

Specifications

• Accession Number: Q62178.2
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 64218
• Molecular Weight (g/mol): M.W.-observed: 90-110 kDa, under reducing conditions., M.W.-theroretical: 99 kDa
• Quantity: 50 μg
• Source: Mouse myeloma cell line, NS0-derived mouse Semaphorin 4 A protein Mouse Sema 4 A (Thr33-Phe683) (C-terminus) Accession # Q62178.2 IEGRMDP Mouse IgG2a (Glu98-Lys330) (N-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: CORD10, RP35, Sema B, sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4 A, SEMA4A, SEMAB, semaphorin-4 A, Semaphorin-B, SemB, SEMBFLJ12287
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 250 μg/mL in PBS.
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Semaphorin 4 A