R&D Systems™ Recombinant Mouse TNF RI/TNFRSF1A Fc Chimera Protein

Catalog No.	Formulation
430RI050	Lyophilized from a 0.2μm filtered solution in PBS with BSA as a carrier protein.
430RI050CF	Lyophilized from a 0.2μm filtered solution in PBS.

Catalog No. 430RI050 Supplier R&D Systems™ Supplier No. 430RI050

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 0.75-4.5 ng/mL in the presence of 0.1 ng/mL recombinant mouse TNF-alpha .

Specifications

• Formulation: Lyophilized from a 0.2μm filtered solution in PBS with BSA as a carrier protein.
• Gene ID (Entrez): 21937
• Molecular Weight (g/mol): 48.6 kDa (monomer)
• Name: TNF RI/TNFRSF1A Fc Chimera
• Quantity: 50 μg
• Source: Mouse myeloma cell line,NS0-derived mouse TNF RI/TNFRSF1A protein, (N-terminus) Mouse TNF RI (Leu30-Ala212) Accession &Num; P25118 DIEGRMD Human IgG1 (Pro100-Lys330) 6-His tag (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Structural Form: Disulfide-linked homodimer
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.