R&D Systems™ Recombinant Mouse Tryptase epsilon/BSSP-4 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Arg-ThioBenzyl ester (Z-R-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468. The specific activity is >5,000 pmol/min/μg, as measured under the described conditions.

Specifications

• Accession Number: Q7TML0
• For Use With (Application): Enzyme Activity
• Formulation: Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
• Gene ID (Entrez): 64063
• Molecular Weight (g/mol): M.W. (Observed): 37 kDa and 33 kDa, reducing conditions; M.W. (theoretical): 32 kDa
• Name: Tryptase epsilon/BSSP-4
• Quantity: 10 μg
• Source: Mouse myeloma cell line, NS0-derived mouse Tryptase epsilon/BSSP-4 protein Ala33-Ser306, with a C-terminal 10-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Structural Form: Pro form
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: Prss22
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 200 μg/mL in sterile 50 mM Tris, 10 mM CaCl2 and 150 mM NaCl, pH 7.5.
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Protein: Tryptase epsilon/BSSP-4/PRSS22