R&D Systems™ Recombinant Mouse Wnt-3/sFRP-1 Complex Protein

Description

Measured by its ability to activate Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 7.00-63.0 ng/mL.

Specifications

• Accession Number: P17553.1, Q8C4U3.3
• Formulation: Lyophilized from a 0.2 μm filtered solution in MOPS and NaCl with Trehalose.
• Gene ID (Entrez): 7473
• Molecular Weight (g/mol): M.W.-observed: 36-45 kDa, under reducing conditions., M.W.-theroretical: 37 kDa (Wnt-3) & 33 kDa (sFRP-1)
• Quantity: 10 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse Wnt-3 protein Mouse Wnt-3 (Gly22-Lys355) (C-terminus) Accession # P17553.1 Mouse sFRP-1 (Ser32-Lys314) (C-terminus) Accession # Q8C4U3.3 (N-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: FrzA, INT4, INT4Proto-oncogene Int-4 homolog, MGC131950, MGC138321, MGC138323, proto-oncogene Wnt-3, SARP-2, sFRP1, TETAMS, wingless-type MMTV integration site family, member 3, WNT3, WNT-3 proto-oncogene protein
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 100 μg/mL in PBS.
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Wnt3