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ACROBiosystems Human APRIL Protein, Active Trimer, His and Flag Tag, 100 μg

Catalog No. 502019606
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Human APRIL (TNFSF13) is a cytokine of the tumor necrosis factor family that binds to receptors TNFRSF13B/TACI and TNFRSF17/BCMA, playing a critical role in the generation and maintenance of mature B-lymphocytes. This active homotrimer protein is expressed in human HEK293 cells and contains a polyhistidine tag followed by a flag tag at the N-terminus. It is lyophilized from a 0.22 μm filtered solution in PBS at pH 7.4 with trehalose as a protectant.

  • Calculated molecular weight of 50.2 kDa with a migration of 55-60 kDa on non-reducing SDS-PAGE due to glycosylation
  • Purity >95% as determined by both SDS-PAGE and SEC-MALS
  • Endotoxin levels <0.01 EU per μg via LAL or rFC method
  • Verified bioactivity for BCMA and TACI binding via ELISA and BLI assays
  • 100% sequence coverage verified by LC-MS/MS peptide mapping

Catalog No. 50-201-9606 Supplier ACROBiosystems Supplier No. APLH52D1100UG
May include imposed supplier surcharges.
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Human APRIL (TNFSF13) is a cytokine of the tumor necrosis factor family that binds to receptors TNFRSF13B/TACI and TNFRSF17/BCMA, playing a critical role in the generation and maintenance of mature B-lymphocytes. This active homotrimer protein is expressed in human HEK293 cells and contains a polyhistidine tag followed by a flag tag at the N-terminus. It is lyophilized from a 0.22 μm filtered solution in PBS at pH 7.4 with trehalose as a protectant.

  • Calculated molecular weight of 50.2 kDa with a migration of 55-60 kDa on non-reducing SDS-PAGE due to glycosylation
  • Purity >95% as determined by both SDS-PAGE and SEC-MALS
  • Endotoxin levels <0.01 EU per μg via LAL or rFC method
  • Verified bioactivity for BCMA and TACI binding via ELISA and BLI assays
  • 100% sequence coverage verified by LC-MS/MS peptide mapping

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