Recombinant Protein;1MG;Human GUCY2C, Fc Tag (GUC-H5257) is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Gln 430 (Accession # P25092-1).;This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 72.5 kDa. The protein migrates as 80-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Human GUCY2C, Fc Tag (Cat. No. GUC-H5257) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human GUCY2C Antibody, Human IgG1 with a linear range of 0.3-5 ng/mL (QC tested).;2e5 of anti-GUCY2C CAR-293 cells were stained with 100 µL of 1 µg/mL Human GUCY2C, Fc Tag (Cat. No. GUC-H5257) and negative control protein respectively, washed and then followed by PE anti-human IgG Fc and analyzed with FACS (Routinely tested).