Recombinant Protein;1MG;Mouse CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag (MALS verified) (CDD-M5219) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 108 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # P22646-1 (CD3E) & P04235-1 (CD3D)).;Mouse CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag is produced by co-expression of CD3E and CD3D, has a calculated MW of 15.1 kDa (CD3E) and 14.3 kDa (CD3D). Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3D is fused with a flag tag at the C-terminus. The reducing (R) heterodimer protein migrates as 19 kDa and 25-33 kDa due to glycosylation.;Immobilized Mouse CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag (Cat. No. CDD-M5219) at 2 μg/mL (100 μL/well) can bind CD3E Monoclonal Antibody (145-2C11), Biotin with a linear range of 0.2-6 ng/mL (QC tested).