Recombinant Protein;20UG;Biotinylated Human CD20 Full Length, His,Avitag (CD0-H82E3) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Pro 297 (Accession # P11836-1).;Nanodiscs are a new class of model membranes that are being used to solubilize and study a range of integral membrane proteins (mp,full length CD20) and membrane-associated proteins. The Nanodisc bilayer is bounded by a membrane scaffold protein (MSP1D1) coat that confers enhanced stability and a narrow particle size distribution.
The CD20 nanodisc assembles from a mixture of full length CD20 protein in detergent, phospholipid micelles and membrane scaffold protein(MSP1D1) upon removal of the detergent.
The CD20 carries a polyhistidine tag at the C-terminus followed by an Avi tag with calculated MW of 36.9 kDa and migrates as 43 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. The membrane scaffold protein(MSP1D1)has calculated MW of 24.7 kDa, and it migrates as 25 kDa under re