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ACROBiosystems Biotinylated Human CTLA-4 Protein, His,Avitag™ (MALS verified)

Supplier:  ACROBiosystems CT4H82E325UG

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Recombinant Protein;25UG;Biotinylated Human CTLA-4, His,Avitag (CT4-H82E3) is expressed from human 293 cells (HEK293). It contains AA Ala 37 - Phe 162 (Accession # Q6GR94-1).;This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 17.2 kDa. As a result of glycosylation, the protein migrates as 22-30 kDa under reducing (R) condition, and 37-45 kDa under non-reducing (NR) condition (SDS-PAGE).;Immobilized Human B7-1, Fc Tag (Cat. No. B71-H5259) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CTLA-4, His,Avitag (Cat. No. CT4-H82E3) with a linear range of 0.7-13 ng/mL (QC tested).;Biotinylated Human CTLA-4, His,Avitag (Cat. No. CT4-H82E3) captured on Biotin CAP - Series S sensor Chip can bind Yervoy (Ipilimumab) with an affinity constant of 0.635 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Catalog No. 50-201-9637


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