Recombinant Protein;25UG;Biotinylated Human 4-1BB, His,Avitag™(MALS verified) (41B-H82E6) is expressed from human 293 cells (HEK293). It contains AA Leu 24 - Gln 186 (Accession # Q07011-1).;This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 20.9 kDa. The protein migrates as 30-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Human 4-1BB Ligand (71-254), His,Flag Tag (active trimer) (MALS verified) (Cat. No. 41L-H52D4) at 1 μg/mL (100 μL/well) can bind Biotinylated Human 4-1BB, His,Avitag™(MALS verified) (Cat. No. 41B-H82E6) with a linear range of 1-39 ng/mL (QC tested).;Immobilized Biotinylated Human 4-1BB, His,Avitag™(MALS verified) (Cat. No. 41B-H82E6) at 1 μg/mL (100 μL/well) on Recombinant Streptavidin (Cat. No. STN-N5116) precoated (0.5μg/well) plate, can bind Human 4-1BB Ligand, Fc Tag (Cat. No. 41L-H5257) with a linear range of 0.1-2 ng/mL (Routinely tested).