The Human CD3E and CD3D Heterodimer Protein is a recombinant, single-pass type I membrane protein complex expressed in human 293 cells. As a key component of the T cell receptor-CD3 complex, this heterodimer plays a crucial role in coupling antigen recognition to intracellular signal-transduction pathways. This product is provided as a lyophilized powder and has been verified via mass spectrometry and SEC-MALS for quality assurance.
- Greater than 90% purity as determined by SDS-PAGE and greater than 95% by SEC-MALS
- Low endotoxin levels (less than 0.01 EU per μg)
- Consists of CD3E (Asp 23-Asp 126) and CD3D (Phe 22-Ala 105)
- Subunit CD3E contains a C-terminal polyhistidine tag, while CD3D is tag-free
- Validated for binding activity via ELISA and SPR assays
- Stable for 24 months when stored lyophilized at -20°C to -70°C