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R&D Systems™ Recombinant Rat B7-H4 Fc Chimera Protein

Catalog No. 10085B7050
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10085B7050 50 μg
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Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED50 for this effect is 1-6 μg/mL.

Specifications

Accession Number Q501W4
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Gene ID (Entrez) 79679
Molecular Weight (g/mol) M.W. (Observed): 74-85 kDa, reducing conditions; M.W. (theoretical): 52 kDa
Quantity 50 μg
Source Mouse myeloma cell line, NS0-derived rat B7-H4 protein Rat B7-H4 (Phe29-Gly257) (N-terminus) Accession # Q501W4 IEGRMDP Mouse IgG2a (Glu98-Lys330) (C-terminus)
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
Regulatory Status RUO
Endotoxin Concentration <0.10 EU / 1 μg of the protein by the LAL method.
Specific Reactivity B7-H4
Conjugate Unconjugated
Species Rat
Recombinant Recombinant
Reconstitution Reconstitute at 500 μg/mL in PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein B7-H4
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