R&D Systems™ Recombinant Rat GDNF Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity, Binding Activity

Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. The ED50 for this effect is 2-12 ng/mL in the presence of Recombinant Rat GFR alpha-1/GDNF R alpha-1 Fc Chimera (Catalog # 560-GR ). Measured by its binding ability in a functional ELISA. Immobilized Recombinant Rat GFR alpha-1/GDNF R alpha-1 Fc Chimera (Catalog # 560-GR ) at 1 μg/mL binds Recombinant Rat GDNF with an apparent Kd <4 nM.

Specifications

• Accession Number: Q07731
• Characterization: Bioactivity, Binding Activity
• For Use With (Application): Bioactivity, Binding Activity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Gene ID (Entrez): 2668
• Molecular Weight (g/mol): M.W. (theoretical): 15 kDa (monomer)
• Quantity: 1 mg
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived rat GDNF protein Ser78-Ile211
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.01 EU / 1 μg of the protein by the LAL method.
• Gene Alias: Astrocyte-derived trophic factor, ATF, ATF1, ATF2, glial cell derived neurotrophic factor, glial cell line derived neurotrophic factor, glial cell line-derived neurotrophic factor, HFB1-GDNF, hGDNF, HSCR3
• Gene Symbol: Gdnf
• Product Type: Recombinant Protein
• Conjugate: Unconjugated
• Species: Rat
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS containing (at least 0.1% human or bovine serum albumin.)
• Purity or Quality Grade: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Protein: GDNF