R&D Systems™ Recombinant S. pyogenes CRISPR-associated Protein 9

Description

Purified CAS9 with Nuclear Localization Sequence

Measured by its ability to cleave a targeted DNA substrate. S. pyogenes CRISPR-Cas9 achieves >80% substrate cleavage, as measured under the described conditions.

Specifications

• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris, NaCl, EDTA, Glycerol and TCEP.
• Gene ID (Entrez): 901176
• Molecular Weight (g/mol): M.W. (Observed): 133 kDa, reducing conditions; M.W. (theoretical): 164 kDa
• Quantity: 50 μg
• Source: E. coli-derived s. pyogenes CRISPR-Cas9 protein APKKKRKVGIHGVPAA S. pyogenes CRISPR-Cas9 (Asp2-Asp1368) (N-terminus) Accession # Q99ZW2 KRPAATKKAGQAKK-KKGYGRKKRRQRRRG HHHHHH (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
• Regulatory Status: RUO
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Cross Reactivity: CRISPR-Cas9
• Species: S. pyogenes
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: CRISPR-Cas9