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R&D Systems™ Recombinant SARS-CoV-2 B.1.1.7 N501Y S RBD Biotin Protein CF

Catalog No. BT10730050
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50 μg
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BT10730050 50 μg
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Description

501Y Mutation found in Alpha, Beta, and Gamma Variants, Biotinylated via Amines

Measured by its binding ability in a functional ELISA with Recombinant Human ACE-2 Fc Chimera (Catalog # 10544-ZN ).

Specifications

Accession Number YP_009724390.1
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Molecular Weight (g/mol) M.W.-observed: 30-40 kDa, under reducing conditions, M.W.-theroretical: 26 kDa
Quantity 50 μg
Source Human embryonic kidney cell, HEK293-derived sars-cov-2 Spike RBD protein Arg319-Phe541 (Asn501Tyr), with a C-terminal 6-His tag
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
Endotoxin Concentration <1.0 EU / 1 μg of the protein by the LAL method.
Conjugate Biotin
Reconstitution Reconstitute at 500 μg/mL in PBS.
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein Spike RBD
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