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Enzo Life Sciences SEEBRIGHT Red 580 dUTP (lyophilized) (50nmol)
Supplier: Enzo Life Sciences ENZ42844L0050
Red 580 [5-ROX] dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, random prime labeling, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Red 580 dUTP can also be used for multicolor fluorescence labeling. This labeled dUTP can be used with the Nick Translation DNA Labeling System 2.0 (ENZ-GEN111). Alternative name: 5-ROX dUTP. Quantity: Sufficient for approximately 98 reactions, following the recommended protocol of ENZ-GEN111. Purity: ≥93% (HPLC). Formulation: Lyophilized.
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