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Thermo Scientific™ Remel™ BactiDrop™ Kovac's Indole
GSA_VA

Catalog No. r21522
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R21522 50 x 0.75 mL
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Catalog No. R21522 Supplier Thermo Scientific™ Supplier No. R21522
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Description

Determine of the ability of microorganisms, to split indole from tryptophan using BactiDrop™ Kovac's Indole reagent.

Determine of the ability of microorganisms, primarily Enterobacteriaceae, to split indole from the tryptophan molecule with Thermo Scientific™ Remel™ BactiDrop™ Kovac's Indole reagent. Earlier tests to confirm indole production required a xylene extraction procedure to detect small quantities of indole produced from the breakdown of tryptophan. Kovac's utilized amyl alcohol instead of ethyl alcohol to bypass the extraction procedure involved in the Ehrlich-Beohme reagent1. Gadebusch and Gabriel reported that substitution of isoamyl alcohol resulted in greater stability of Kovac's reagent2.

Tryptophanases are intracellular enzymes that mediate indole production by hydrolysing the amino acid tryptophan. Indole combines with dimethyl-aminobenzaldehyde to form a red compound. This is a condensation product formed by an acid splitting of a protein3.Ready-to-use: Liquid reagentEasy to interpret: Positive Test - A pink-red ring between the broth and reagentNegative Test - No color developmentKovac's Indole Reagent contains 5% w/v ρ-dimethylaminobenzaldehyde (CAS 100-10-7), hydrochloric acid (concentrated) (CAS 7647-01-0) and amyl alcohol (CAS 71-41-0) and is supplied as 0.75 mL ampules..Not all products are available for sale in all territories. Please inquire.Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.

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Specifications

Content And Storage 20°C to 25°C
Description BactiDrop Indole, Kovacs
CE Marker Yes
Detectable Analytes Nitrite, Free Nitrogen Gas
Quantity 50 x 0.75 mL
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