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Thermo Scientific™ Decarboxylase Broth (Moeller) Lysine
Description
Differentiate gram-negative enteric bacilli based on the ability of an organism to decarboxylate lysine using Decarboxylase Broth (Moeller) Lysine.
Differentiate gram-negative enteric bacilli based on the enzymatic ability of an organism to decarboxylate lysine using Thermo Scientific™ Remel™ Decarboxylase Broth (Moeller) Lysine. In 1955, Moeller developed the amino acid decarboxylase media to detect production of lysine and ornithine decarboxylase and arginine dihydrolase1. These media are used to differentiate bacterial groups within the Enterobacteriaceae2. Ewing, Davis, and Edwards reported Moeller's formulation to be more reliable for differentiating Klebsiella and Enterobacter as compared to other media tested3.
The Moeller method is considered the standard or reference method for determining the decarboxylase reactions of the Enterobacteriaceae. This medium helps differentiate gram negative bacilli by their ability to decarboxylate lysine. The lysine decarboxylase test is used to differentiate between Salmonella and Shigella.
- Recommended as the standard or reference method for determining decarboxylase reactions of Enterobacteriaceae.
- Ready to Use: Convenience of prepared media.
- Ease of Identification: Visual detection of color change.
This liquid medium is recommended for use in qualitative procedures. This basal medium contains meat peptone and beef extract that provide the nitrogenous nutrients required for bacterial growth. Dextrose acts as a source of energy and fermentable carbohydrate. Bromcresol purple and cresol red are pH indicators. Pyridoxal is an enzyme cofactor which enhances decarboxylase activity4. Lysine is an amino acid that is added to the basal medium to detect the production of enzymes which decarboxylase lysine.
When an organism ferments dextrose, acids are produced which lower the pH, resulting in a color change from purple to yellow. If decarboxylation of lysine occurs in response to the acid pH, the alkaline end-product cadaverine (amine) is formed which results in the medium reverting to its original color (purple).
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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.
Specifications
Specifications
| Description | Decarboxylase Broth Lysine (Lysine Broth) Moeller |
| Format | D - 5mL |
| Product Type | Microbiology Media |
| Quantity | 20/Pk. |
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