Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Microbiological Media and Media Additives
  4. Prepared Microbiology Media
  5. R060856

Thermo Scientific™ Dubos Polysorbate 80 Albumin Broth

Catalog No. R060856
This item is not returnable. View return policy
This item is not returnable. View return policy

Description

Cultivate Mycobacterium species with Dubos Polysorbate 80 Albumin Broth.

Cultivate Mycobacterium species with Thermo Scientific™ Remel™ Dubos Polysorbate 80 Albumin Broth.

In 1946, Dubos and Davis described a liquid medium in which growth of tubercle bacillus was obtained from dilute inocula in 3 to 5 days1. Dubos and Middlebrook2 then studied the use of this medium to cultivate Mycobacterium tuberculosis. The original solution was modified in 1950 by Dubos, Fenner and Pierce3 by adding a strong buffering system and an acid pH which provided dispersed growth from a small inoculum. This broth is used for subculturing mycobacteria and preparing inoculum for other in vitro tests4.

Dubos Polysorbate 80 Albumin Broth is a liquid medium recommended for cultivation of Mycobacterium species. Polysorbate 80 is an oleic acid ester and an in vitro indicator of mycobacterial virulence. Virulent cultures form cords in media containing oleic acid or its esters, while nonvirulent cultures grow more diffusely.

  • Ready-to-use: Convenience of prepared media.

Dubos Polysorbate Albumin Broth contains casein peptone and asparagine that supply nitrogen for the growth of mycobacteria. Disodium and monopotassium phosphates provide the buffering system. The inorganic salts supply ions essential for metabolism. Polysorbate 80 (10%) serves as a nutritional source of essential fatty acids and also acts as a dispersing agent for homogenous growth from a small inoculum. Bovine plasma albumin (serum factor V) enhance the growth of mycobacteria and provides protection against bacteriostatic and bactericidal effect by toxic agents.

 

Not all products are available for sale in all territories. Please inquire.

Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.

TRUSTED_SUSTAINABILITY

Specifications

Description Dubos Polysorbate Albumin Broth
Format D - 5mL
Product Type Microbiology Media
Quantity 100/Pk.
Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Your feedback has been submitted: Thank you for helping us improve our website.