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Thermo Scientific™ Remel™ Ehrlich's Indole Reagent

Catalog No. R21213
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Quantity:
25 mL
This item is not returnable. View return policy
This item is not returnable. View return policy

Description

Detect indole production by microorganisms due to tryptophanase activity with Ehrlich's Indole Reagent.

Detect indole production by nonfermenters and anaerobes grown in indole broth with Thermo Scientific™ Remel™ Ehrlich's Indole Reagent.Ehrlich's reagent contains p-dimethylaminobenzaldehyde that acts as an indicator to identify indoles. The use of ρ-dimethylaminobenzaldehyde in the indole test was first reported by Vracko and Sherris1. Ehrlich's Indole Reagent is recommended for detecting indole production by nonfermenting gram negative bacilli and for anaerobes2-4.

Use Ehrlich’s Indole Reagent to detect indole production by nonfermenters and anaerobes grown in indole broth.Ready to use: liquid reagent.Easy to interpret:Positive test: pink-red ring between broth and solvent within 15 minutes.Negative test: no color development in the form of a ring within 15 minutes.Tryptophanases are intracellular enzymes that mediate indole production by hydrolysing the amino acid tryptophan. Indole combines with dimethyl-aminobenzaldehyde to form a red compound. This is a condensation product formed by an acid splitting of a protein. When Ehrlich’s reagent is used, small amounts of indole are produced from the breakdown of tryptophan by tryptophanase. These must be extracted first with xylene or a xylene substitute before they are detectable5Ehrlich's Indole Reagent contains ρ-dimethylaminobenzaldehyde (CAS 100-10-7), hydrochloric acid (Concentrated) (CAS 7647-01-0) and 95% ethyl alcohol (CAS 64-17-5). Ehrlich’s Indole Reagent is not specific for indole. When a solvent like xylene is used to extract indole, the red color that develops in the solvent layer occurs only with indole and skatole6. Use of inadequate peptone broth or glucose-containing medium may cause aberrant results6. Not all products are available for sale in all territories. Please inquire.Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.
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Specifications

Description Indole Reagent, Ehrlich's
Detectable Analytes Indole, Skatole, Tryptophanase
Format Bottle
Quantity 25 mL
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