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Thermo Scientific™ KBE (Kanamycin Bile Esculin) Agar

Catalog No. R01510
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R01510 15mm x 103mm Tube
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Catalog No. R01510 Supplier Thermo Scientific™ Supplier No. R01510
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Description

Selectively isolate and presumptively identify Bacteroides fragilis group using KBE Agar (Kanamycin Bile Esculin Agar).

Selectively isolate and presumptively identify Bacteroides fragilis group using Thermo Scientific™ Remel™ KBE Agar (Kanamycin Bile Esculin Agar). Chan and Porschen formulated KBE Agar to selectively isolate and presumptively identify the B. fragilis group based on resistance to bile and kanamycin and hydrolysis of esculin1,2. Clinical specimens for anaerobic culture frequently contain mixed flora, including facultative gram-negative rods that readily overgrow the anaerobes, so selective media are recommended for isolation of anaerobes3.

Members of the Bacteroides fragilis group are the anaerobic bacteria most frequently isolated in human clinical infections4. This group includes B. fragilis, B. thetaiotaomicron, B. ovatus, B. distasonis, B. vulgatus, and others. These members of the B. fragilis group hydrolyze esculin to produce esculetin.

Selectively isolate and presumptively identify members of the B. fragilis group using KBE Agar.

  • Ready to use – Convenience of prepared media
  • Easy to interpret - Brown-black complex in the agar surrounding the colony
  • Selective media - Addition of kanamycin and bile makes this medium selective
    • Kanamycin (1000μg/ml) inhibits facultative anaerobes and anaerobic gram-negative bacteria other than B. fragilis group
    • Bile (20%) stimulates B. fragilis but inhibits other anaerobic bacteria

Ferric ammonium citrate present in the medium reacts with esculetin to produce a brown-black complex in the agar surrounding the colony. Addition of kanamycin and bile to this medium makes it selective. Vitamin K and hemin are growth factors. Hemin also allows testing for catalase production5.

TRUSTED_SUSTAINABILITY

Specifications

Description KBE Agar
Format 15mm x 103mm Tube
Product Type Agar
Quantity 20 x 5 mL Butt
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