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Thermo Scientific™ L-J Gruft
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Catalog No. R08520
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1 oz. Bottle
20mm x 113mm Tube - Slant
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R08520 100/Pk. 20mm x 113mm Tube - Slant
R08522 20/Pk. 20mm x 113mm Tube - Slant
R10103 10/Pk. 1 oz. Bottle
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Catalog No. R08520 Supplier Thermo Scientific™ Supplier No. R08520
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Description

Selectively isolate and cultivate Mycobacterium spp. using Lowenstein-Jensen (L-J) Medium, L-J Gruft, and L-J Mycobactocel.

Selectively isolate and cultivate Mycobacterium spp. using Thermo Scientific™ Remel™ Lowenstein-Jensen (L-J) Medium, L-J Gruft, and L-J Mycobactocel. Medium consisting of congo red and malachite green dyes was formulated by Lowenstein to cultivate mycobacteria1. This medium was modified by Jensen by replacing congo red with citrate and phosphate contents and increasing the malachite green concentration2. L-J Medium used for recovery of pathogenic Mycobacterium spp. was found to be superior to other media tested, including ATS (American Trudeau Society) Medium3,4.

Selectively isolate and cultivate Mycobacterium spp. using Lowenstein-Jensen (L-J) Medium, L-J Gruft, and L-J Mycobactocel.

  • Selective – Antibiotics in the medium make the medium selective for Mycobacterium while inhibiting contaminating bacteria.
  • Ready to use – Convenience of prepared media.

L-J Medium is a glycerated egg-potato medium. The glycerol and egg mixture provide fatty acids and protein required for the metabolism of mycobacteria. L-J Medium was modified by Gruft by adding ribonucleic acid as a growth stimulant and penicillin and nalidixic acid as selective inhibitors of contaminating bacteria5. Ribonucleic acid increases the percentage of positive cultures over the standard L-J Medium. The selectivity of L-J Medium was increased by incorporating cycloheximide, lincomycin, and nalidixic acid and was formulated by Petran and Vera6.

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Specifications

Description Lowenstein-Jensen Gruft. (LJ) w/penicillin, nalidixic acid
Format 20mm x 113mm Tube - Slant
Product Type Prepared Microbiology Tubes
Quantity 100/Pk.
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