Thermo Scientific™ Middlebrook 7H9 Broth w/Polysorbate 80

Description

Differentiate and identify Mycobacterium species based on their ability to reduce potassium tellurite using Middlebrook 7H9 Broth w/Polysorbate 80.

Differentiate Mycobacterium species based on their ability to reduce potassium tellurite using Thermo Scientific™ Remel™ Middlebrook 7H9 Broth w/Polysorbate 80 (Tellurite Reduction Medium). Middlebrook and Cohn developed Middlebrook 7H9 Broth in 1958 to cultivate mycobacteria¹. Kilburn et al. described the differential identification of mycobacteria using the tellurite reduction test². They reported that all mycobacteria growing actively on a solid medium rapidly reduced a solution of tellurite salt used to flood the plate.

Use Middlebrook 7H9 Broth w/Polysorbate 80 for differentiation of Mycobacterium species based on their ability to reduce potassium tellurite.

• Ready-to-use – Convenience of prepared media.
• Easy-to-interpret – Based on tellurite reduction.

Middlebrook 7H9 Broth w/Polysorbate 80 contains inorganic compounds that supply potassium, sulfur, magnesium, and phosphorus necessary for the growth of mycobacteria. Inorganic copper, iron, zinc, and calcium are growth stimulators. Sodium citrate holds certain inorganic cations in solutions. Ammonium sulfate is a source of nitrogen and dextrose is a source of carbon. Albumin binds to the free fatty acids which may be toxic to Mycobacterium species and acts as a protective agent. Catalase destroys toxic peroxides which may be present in the medium and catalyzes the reaction of iron with molecular oxygen. Biotin is required for carboxylation and decarboxylation reactions and pyridoxine is a precursor of enzymatic activity. Polysorbate 80 is a wetting agent that encourages more homogenous growth.

Specifications

• Description: Middlebrook 7H9 Broth with Polysorbate 80 Tellurite Reduction Medium
• Format: 20mm x 150mm Tube
• Product Type: Microbiology Media
• Quantity: 20 x 5 mL

Safety and Handling

SDS