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  5. Thermo Scientific™ Modified EMB (HHT) Agar w/Lactose, Sucrose

Thermo Scientific™ Modified EMB (HHT) Agar w/Lactose, Sucrose

Catalog No. R01406
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Description

Isolate and differentiate gram-negative enteric bacilli from clinical and industrial samples with Modified (HHT) EMB Agar w/Lactose, Sucrose.

Isolate and differentiate gram-negative enteric bacilli from clinical specimens with Thermo Scientific™ Remel™ Modified EMB (HHT) Agar w/Lactose, Sucrose.

Holt-Harris and Teague1 developed Eosin Methylene Blue Agar (EMB Agar). The combination of dyes, lactose and sucrose incorporated in the medium provides a differential plating medium which helps to identify fermentative, gram-negative bacilli.

Modified (HHT) EMB Agar w/Lactose, Sucrose is a solid medium used to differentiate gram-negative enteric bacilli.

  • Ready-to-use: Convenience of prepared media
  • Easy-to-read: Visible color change due to biochemical reaction
  • Selective medium: Eosin and methylene blue dye selectively inhibit the growth of gram-positive organisms
  • Conforms to CLSI standards2

Modified (HHT) EMB Agar w/Lactose, Sucrose contains gelatin peptone that supplies amino acids and essential nutrients necessary for the growth of bacteria. Lactose fermenting coliform and lactose nonfermenters are distinguished based on different colored colonies formed. Eosin and methylene blue dyes also act as selective agents to inhibit the growth of gram-positive organisms.

The characteristic of certain coliforms to ferment lactose or sucrose can facilitate their identification and differentiation. Eosin dye combines with methylene-blue indicator to produce a color change when lactose or sucrose is fermented. Lactose fermenters such as Escherechia coli produce blue-black colonies with a green metallic sheen due to amide bonding of dye in an acidic environment. Other coliforms form mucoid, pink-brown colonies in less acidic conditions, whereas lactose nonfermenters Shigella and Salmonella form transparent, colorless or amber colored colonies.

Not all products are available for sale in all territories. Please inquire.

Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.

TRUSTED_SUSTAINABILITY

Specifications

Description EMB Agar, Modified (HHT) w/Lactose, Sucrose
Format 12mm x 85mm Monoplate
Product Type Agar
Quantity 10/Pk.
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