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Thermo Scientific™ MRVP Broth
Identify enteric gram-negative bacilli using MR-VP Broth.
$274.00 - $1556.00
Specifications
Certifications/Compliance | Industrial Reference: BAM, SMWW, USDA. |
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Description | MR-VP Broth |
Format | 15mm x 103mm Tube |
Product Type | Microbiology Media |
Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
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Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
R061432
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Thermo Scientific™
R061432 |
20/Pk. |
Pack of 20 for $279.00
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R061436
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Thermo Scientific™
R061436 |
20 x 5 mL |
Pack of 20 for $274.00
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R061430
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Thermo Scientific™
R061430 |
100 x 2 mL |
Case of 100 for $1,556.00
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R061434
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Thermo Scientific™
R061434 |
100 x 5 mL | Item Discontinued This item has been discontinued by the supplier. Please Sign In to view product availability in your area. | N/A | |||||
Description
Identify and differentiate amongst coli-aerogene groups using Thermo Scientific™ Remel™ MR-VP Broth. In 1898, Voges and Proskauer reported the production of a red color after the addition of potassium hydroxide to cultures grown on an appropriate medium1. Later Barritt made the VP test more sensitive by addition of alpha naphthol to the medium2. In 1915, Clarke and Lubs first described the methyl red test in differentiating between the coli-aerogenes group of enteric bacteria3. Further MR-VP broth was developed to permit both tests to be performed using the same medium.
All members of Enterobacteriaceae are, by definition, glucose fermenters, resulting in the production of acid. MR-VP Broth is recommended for use in qualitative procedures for the performance of Methyl Red (MR) and Voges-Proskaur (VP) test as an aid in the identification of enteric gram-negative bacilli.
- Both MR and VP tests can be performed in the same medium.
- Easy-to-differentiate: MR positive organisms show a red color development and VP positive organisms produce a pink-red color development, while MR and VP negative organisms produce yellow color on the surface of the medium.
- Ready-to-use: Convenience of prepared media.
The MR test is based on the use of a pH indicator, methyl red, to determine the hydrogen ion concentration present when an organism ferments glucose4. This medium contains buffered peptone water, which supports growth of organisms. MR-positive organisms continue to produce acids, overcoming the phosphate buffer and resulting in a low pH and a red color development. MR-negative organisms further metabolize the initial fermentation products by decarboxylation to produce neutral acetyl methyl carbinol (acetoin), which results in a neutral pH.
The VP test is based on the detection of neutral end-product acetylmethylcarbinol derived from glucose metabolism and is used primarily to separate Escherichia coli (VP negative) from the Klebsiella-Enterobacter group (VP positive). Acetylmethylcarbinol is a precursor to 2,3-butanediol, both of which are oxidized to diacetyl which is the reactant for the pink-red color produced in the VP test5.
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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.
Specifications
Industrial Reference: BAM, SMWW, USDA. | |
MR-VP Broth | |
15mm x 103mm Tube | |
Microbiology Media |
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