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Thermo Scientific™ PPLO Agar

Catalog No. R20260
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Quantity:
10/Pk.
Format:
15mm x 60mm Plate
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Catalog No. Quantity Format
R20260 10/Pk. 15mm x 60mm Plate
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Catalog No. R20260 Supplier Thermo Scientific™ Supplier No. R20260
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Description

Selectively isolate and cultivate Mycoplasma species using PPLO Agar (w/horse serum, amphotericin B, penicillin, thallium acetate).

Selectively isolate and cultivate Mycoplasma species using Thermo Scientific™ Remel™ PPLO Agar (w/horse serum, amphotericin B, penicillin, and thallium acetate). Pleuropneumonia-like organisms (PPLO) were first studied and determined to be the causative agent of bovine pleuropneumonia. Edward and Freundt proposed the recent system of classification and nomenclature for PPLO, now referred to as Mycoplasmataceae1,2. Kraybill confirmed that M. pneumoniae is resistant to methylene blue in agar medium while other species of Mycoplasma are inhibited3,4.

The pleuropneumonia-like organisms (PPLO) are a group of pleomorphic, filterable, fastidious microorganisms which also lack a rigid cell wall. PPLO agar base was formulated to isolate and cultivate Mycoplasma species5. Addition of yeast extract and unheated horse serum modified this formulation6.

  • Selective medium – Addition of penicillin and thallium acetate in the medium inhibit bacteria, amphotericin B inhibits yeasts and molds and methylene blue inhibits species of Mycoplasma other than pneumoniae.
  • Ready-to-use – Convenience of prepared medium

This medium contains beef extract infusion and peptone which supply nutrients that are necessary for growth of mycoplasmas. Sodium chloride maintains osmotic equilibrium. Yeast extract enhances the growth by providing B-complex. Normal horse serum provides a protein source.

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.

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Specifications

Description PPLO Agar w/horse serum, amphotericin B, penicillin, thallium acetate
Format 15mm x 60mm Plate
Product Type Agar
Quantity 10/Pk.
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