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Thermo Scientific™ Regan Lowe Semi-Solid Transport

Catalog No. R064141
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20 x 5 mL
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R064141 20 x 5 mL
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Catalog No. R064141 Supplier Thermo Scientific™ Supplier No. R064141
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Description

Transport and selectively isolate Bordetella pertussis and Bordetella parapertussis using Regan-Lowe Semi-Solid Transport Medium.

Selectively isolate Bordetella pertussis and Bordetella parapertussis with Thermo Scientific™ Remel™ Regan-Lowe Semi-Solid Transport Medium. Regan and Lowe demonstrated that charcoal agar supplemented with 40μg/mL cephalexin and 10% defibrinated horse blood was an excellent enrichment and transport medium1. Hoppe reported that horse blood was superior to sheep or human blood for recovery of Bordetella spp. and incubation in ambient air results in more luxuriant growth than incubation in 5-10% CO22.

B. pertussis and B. parapertussis that cause whooping cough, are amongst the most fastidious bacteria known and require special culturing techniques. Regan-Lowe transport medium is semi-solid in nature and is used for transportation and selective isolation of Bordetella pertussis and Bordetella parapertussis. The medium contains Cephalaxin which inhibits penicillin-resistant staphylococci, and some coliforms, as well as strains of Haemophilus influenzae and allows selective isolation of Bordetella spp. from respiratory specimens.

  • Ready-to-use – Prepared media in 15mm x 103mm tubes (5mL)
  • Selective – Cephalexin and Amphotericin B antibiotics inhibit penicillin resistant staphylococci, coliforms, Haemophilus influenzae; yeasts and fungi respectively and provides selective isolation of Bordetella spp.

Regan-Lowe Semi-Solid Transport Medium contains charcoal agar base with 10% lysed horse blood which provides an enriched medium. Nicotinic acid is added as an essential nutrient for Bordetella. Horse blood acts as a growth factor for B. pertussis and as a neutralizer of inhibiting substances2. Cephalexin inhibits penicillin resistant staphylococci, coliforms and, Haemophilus influenzae. Amphotericin B inhibits growth of yeasts and fungi.

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.

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Specifications

Description Regan-Lowe Semi-Solid Transport Medium
Format 15mm x 103mm Tube
Product Type Microbiology Media
Quantity 20 x 5 mL
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