Learn More
Thermo Scientific™ RPMI 1640 Agar w/MOPS and 2% Glucose
Description
Determine susceptibility testing of Candida spp. directly from colonies grown on non selective media using RPMI 1640 Agar w/MOPS and 2% Glucose.
Determine susceptibility to antifungal agents by testing Candida spp. directly from colonies grown on non-selective media with Thermo Scientific™ Remel™ RPMI 1640 Agar w/MOPS and 2% Glucose. RPMI 1640 was developed by Moore et al. and is based on RPMI-1630 series of media utilizing a bicarbonate buffering system. When supplemented with morpholinepropanesulfonic acid (MOPS), glucose and agar, RPMI-1640 has demonstrated accuracy for use with gradient-based systems with results comparable to that obtained with the CLSI reference method for testing Candida spp. against antifungal agents1-7.
Invasive fungal infections are of great clinical relevance and pose therapeutic challenges due to increasing resistance to the most commonly prescribed antifungals. Concerns of emerging resistance and the introduction of new antifungal agents have led to increased demand for accurate species identification and antifungal susceptibility testing.
RPMI 1640 Agar w/MOPS and 2% glucose is a plated medium that is used with antibiotic gradient-based systems for susceptibility testing of Candida spp. which causes nonsocomical bloodstream infections (BSI) in the United States8,9.
RPMI-1640 medium is widely used in cell culture and also as a reference method for antifungal broth microdilution recommended by CLSI. The use of RPMI-1640 medium supplemented with MOPS, agar, and glucose in the yeast susceptibility test in conjunction with antifungal gradient-based systems has been widely published and suggested as a reliable alternative to traditional broth dilution since the 1990s.
- Easy-to-use – Preformed, stable antibiotic gradient strip is used.
- Easy-to-read – After incubation a symmetrical inhibition ellipse centred along the carrier is seen and the zone edge intersects the strip at the MIC value given in μg/ml.
- Results comparable to the Clinical and Laboratory Standards Institute (CLSI) reference method for antifungal broth microdilution.
RPMI 1640 Agar w/MOPS and 2% Glucose contains MOPs, glucose, RPMI-1640, agar and demineralized water. This medium uses gradient-based systems. The gradient method is based on a combination of the concepts of both dilution and diffusion tests, but differs from conventional disk methods. When the strip is applied to the inoculated agar plate , there is an immediate release of the antibiotic into the agar matrix. A continuous and exponential gradient of antibiotic concentration is created beneath the carrier.
Not all products are available for sale in all territories. Please inquire.
Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.
Specifications
Specifications
| Description | RPMI 1640 Agar w/ MOPS and 2% Glucose |
| Format | 15mm x 150mm Plate |
| Product Type | Agar |
| Quantity | 10/Pk. |
Your input is important to us. Please complete this form to provide feedback related to the content on this product.